Table 2

Comparison of intracellular solutions

Compound

Mammalian R-solution

KCl

MiR05

Fish R-solution


CaK2EGTA

2.77

K2EGTA

7.23

Imidazole

20

K-MES

100

KCl

125

20

Sucrose

110

K-lactobionate

60

85

MgCl2

1.38

3

3

3

K2HPO4/KH2PO4

3

5

10

5

Taurine

20

20

20

Dithiothreitol

0.3

0.3

0.3

EGTA

0.4

0.5

0.4

Hepes

20

20

20

Glutamate

5

5

5

5

Malate

2

2

2

2

BSA

2 mg/ml

2 mg/ml

1 mg/ml

2 mg/ml

pH

7.1

7.1

7.1

7.1

Ionic strength

142

142

95

122

Osmolarity

288

323

330

301


Composition of the mammalian R-solution, KCl-solution (Oroboros, Austria), mitomed MiR05 (Oroboros, Austria) used for mammalian cardiomyocytes and the fish R-solution developed for the present study on permeabilized trout cardiomyocytes. All units are in mM except osmolarity, which is in mOsm.

Sokolova et al. BMC Cell Biology 2009 10:90   doi:10.1186/1471-2121-10-90

Open Data