Table 1

Replication factory numbers determined by confocal and STED microscopy.

Confocal

STED


Mean

Range

Mean

Range


PCNA -HU

1.6

1.2 - 2.1

4.1

2.1 - 5.4


RPA -HU

2.8

1.0 - 4.1

4.7

3.0 - 7.6


PCNA +HU

1.7

1.4 - 1.8

2.6

1.5 - 3.5


RPA +HU

1.8

1.2 - 2.6

2.8

1.9 - 3.9


Cells treated with HU or untreated were analysed for RPA or PCNA containing foci by indirect immunofluorescence using confocal or STED microscopy. The images were restored using the CMLE algorithm and rendered in 3D using SVI's Huygens software, which was also used to count the objects. The average number of objects per μm3 (n = 3-5) and the range of values found are given for a garbage threshold volume (below which size objects are not counted) of 5 voxels (corresponding to 0.0003 μm3).

Cseresnyes et al. BMC Cell Biology 2009 10:88   doi:10.1186/1471-2121-10-88

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