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Open Access Research article

Global transcriptional response after exposure of fission yeast cells to ultraviolet light

Henriette C Skjølberg12, Øyvind Fensgård3, Hilde Nilsen3, Beáta Grallert12 and Erik Boye12*

Author Affiliations

1 Department of Cell Biology, Institute for Cancer Research, Oslo University Hospital, Radiumhospitalet, Montebello, 0310 Oslo, Norway

2 Institute for Molecular Biosciences, University of Oslo, Blindernveien 31, 0371 Oslo, Norway

3 The Biotechnology Center, University of Oslo, Gaustadalleen 21, 0349 Oslo, Norway

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BMC Cell Biology 2009, 10:87  doi:10.1186/1471-2121-10-87

Published: 16 December 2009

Additional files

Additional file 1:

A schematic presentation of the experimental design. Exponentially growing cells were synchronised by a four-hour temperature shift to 36°C. For the time-course experiment cells were UVC-irradiated when shifted back to the permissive temperature and control or irradiated cells were harvested at the time points indicated (black dots). For the restrictive-temperature experiment cells were UVC-irradiated at 36°C after synchronisation, held at the restrictive temperature and control or irradiated cells were harvested at the time point indicated (black dot).

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Additional file 2:

Flow cytometry histogram from the time-course experiment. Flow cytometry histograms of control (C) and UVC-irradiated (UVC) G1-synchronised cells incubated for the times indicated (in minutes) after exposure.

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Additional file 3:

UV-repressed genes that are not CESR genes. 44 genes that were repressed more than twofold were indentified and almost all of them (40) were non-CESR genes. These 40 genes were categorised according to the function of their products.

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Additional file 4:

172 genes changed in the time-course experiment by UV-irradiation. The cell-cycle-regulated genes were excluded from the 241 genes that were up- or downregulated after UVC, in order to identify the UVC-specific transcripts, resulting in the 172 UVC-regulated genes shown here.

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Additional file 5:

Enriched gene ontology groups in the restrictive-temperature experiment. Gene ontology (GO) enrichment analysis was performed on the 43 UVC-induced genes in the restrictive-temperature experiment (Table 1) using the DAVID software after ID-conversion. Ten unique genes (20%) were members of enriched GO groups.

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Additional file 7:

Genes induced in the time-course experiment: protein-protein interactions. Gene products of the regulated genes from the timecourse experiment form an interconnected network involving translation and transcription. Protein-protein interactions were analyzed in FunCoup using the corresponding S. cerevisiae orthologues.

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Additional file 6:

Fission yeast homologues of UPR-induced budding yeast genes. We used the data from both the restrictive-temperature and timecourse experiments to investigate whether UPR genes are induced by UVC in fission yeast. We identified UPR-genes in fission yeast as the homologues of the UPR-induced budding yeast genes.

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