Research article
The Golgi α-1,6 mannosyltransferase KlOch1p of Kluyveromyces lactis is required for Ca2+/calmodulin-based signaling and for proper mitochondrial functionality
1 Dpt. Developmental and Cell Biology, University LA SAPIENZA, P.le A. Moro,5 00185 Rome, Italy
2 Pasteur Insitute-Fondazione Cenci Bolognetti, LA SAPIENZA, Rome, Italy
3 Dpt. Experimental Medicine, University LA SAPIENZA, Rome, Italy
4 National Centre for Rare Diseases, Istituto Superiore di Sanità, Rome, Italy
5 Institut de Génétique et Microbiologie, UMR8621, Université Paris-Sud, 91405 Orsay Cedex, France
BMC Cell Biology 2009, 10:86 doi:10.1186/1471-2121-10-86
Published: 14 December 2009Additional files
Additional file 1:
Cell viability after H2O2 challenge. Indicated strains, grown to exponential phase on YPD medium, were challenged with 20 mM H2O2 for 2 h. The viability was evaluated plating the samples on YPD and was expressed as the CFU percentage of the corresponding untreated cultures. The values were the mean of three independent experiments and showed an SD < 10%.
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