Open Access Research article

Disruption of occludin function in polarized epithelial cells activates the extrinsic pathway of apoptosis leading to cell extrusion without loss of transepithelial resistance

Neal E Beeman15, Heidi K Baumgartner1, Patricia G Webb1, Jerome B Schaack23 and Margaret C Neville14*

Author Affiliations

1 Department of Physiology and Biophysics, School of Medicine, University of Colorado Denver, Aurora, CO 80045, USA

2 Department of Microbiology, School of Medicine, University of Colorado Denver, Aurora, CO 80045, USA

3 Program in Molecular Biology, School of Medicine, University of Colorado Denver, Aurora, CO 80045, USA

4 Department of Obstetrics and Gynecology, School of Medicine, University of Colorado Denver, Aurora, CO 80045, USA

5 Dept of Pathology, Emory University, Whitehead Building, Rm 111, 615 Michael St., Atlanta, Georgia 30322, USA

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BMC Cell Biology 2009, 10:85  doi:10.1186/1471-2121-10-85

Published: 9 December 2009

Additional files

Additional file 1:

Effect of LYHY on TUNEL reactivity and Caspase activation (figure). A. Eph4 cells were grown on glass slides and treated with LYHY or control LYQY peptide for 12 hours. Cells were TUNEL stained. B. Mature Eph4 monolayers on glass slides were treated with the LYHY peptide (panels B1-3) or with LYQY (panels B4-6) at 350 μM for 2 hours and stained live for activated caspase 8 (green; carboxyfluorescein-LETD-fluoromethyl ketone) and caspase3 (red; sulphorhodamine-DEVD-fluoromethyl ketone). Yellow regions in panels 1 and 4 represent colocalization. The arrows in panels 1 - 3 indicate regions of caspase 8 activation with no apparent caspase 3 activation.

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