Additional file 1.

Interaction of CLN5 with endogenous NCL proteins in HeLa cells. The mouse Cln5-cDNA was expressed as a GST fusion protein and used for pull down analyses of endogenous NCL proteins from HeLa cell lysates. The bound proteins were immunoblotted and detected with specific antibodies: CLN2, rabbit polyclonal antibody 7951 [23], CLN3, polyclonal 385 antibody [21] PPT1, rabbit polyclonal antibody 8414 [19], and with anti-CLN6 antibody, which was a generous gift from Dr. S. Mole (London, UK) (Wheeler et al. Am J Hum Genet 70, 2002).

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Lyly et al. BMC Cell Biology 2009 10:83   doi:10.1186/1471-2121-10-83