Additional file 3.
hnRNP G represses Sam68-mediated CD44 variable exon v5 inclusion. (A) Minigene pETv5 contains the CD44 variable exon v5 cloned downstream of a constitutively-active RSV promoter. Splicing events are shown by broken lines, and arrows show the location of primers for RT-PCR. (B) HEK293 cells were transfected with the pETv5 minigene (150 ng), and expression vectors for hnRNP G or GFP-Sam68 (500 ng). The gel images are representative of at least three independent experiments, from which densitometric assessment of RT-PCR product was performed to obtain means +/- standard error (shown in bar chart). The lane marked M shows the migration of the 1 Kb plus DNA ladder (Invitrogen). Ectopic expression of hnRNP G protein repressed CD44 variable exon v5 inclusion both in the presence and absence of ectopic Sam68 (compare lane 1 with lane 3, and lane 2 with lane 4).
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Rajan et al. BMC Cell Biology 2009 10:82 doi:10.1186/1471-2121-10-82