Additional file 1.
Sam68 is efficiently immunoprecipitated by its cognate antisera. LNCaP cell nuclear extracts were subjected to immunoprecipitation (IP) using anti-Sam68 rabbit antisera. IP was carried out in the presence or absence (+/-) of antisera to Sam68 or normal rabbit IgG (negative control), with or without (+/-) Dynabeads Protein A, and either with or without (+/-) prior cross-linking of the antisera to Sam68 to the Dynabeads Protein A. Recovered material was subjected to Western analysis with the antisera to Sam68. Western analysis confirmed that Sam68 protein was efficiently immunoprecipitated by its cognate antisera, and that this was most efficient if the antisera were cross-linked to the Dynabeads Protein A prior to incubation with nuclear extracts (compare lanes 3 and 4). Sam68 protein was not pulled down by Dynabeads Protein A alone (lane 2).
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Rajan et al. BMC Cell Biology 2009 10:82 doi:10.1186/1471-2121-10-82