Additional file 6.
Purification of tau and its mutants. Six truncated tau isolation and deletion mutants were constructed. Primers used were as shown in Additional file 1. Mutants were constructed as indicated (Figure 4). Mutants were expressed in E. coli and then purified through a Ni-NTA column. Samples were electrophoresed on a Tris-Tricine gel (panel b). Western blotting of tau mutants using monoclonal anti-His antibodies (panel b). Tau purified by Q-Sepharose and SP-Sepharose chromatography was analyzed by SDS-PAGE and western blotting using tau-13 anti-tau monoclonal antibodies (panel a).
Format: JPEG Size: 71KB Download file
He et al. BMC Cell Biology 2009 10:81 doi:10.1186/1471-2121-10-81