High speed co-sedimentation assay of tauPRD and F-actin. F-actin (at a constant concentration) was incubated with different concentrations of tauPRD (panel a), tauPRD alone (panel b) and tau (panel c) in the binding buffer without addition of extra NaCl at 37°C for 40 min and then centrifuged (100,000 g, 4°C, 60 min). The pellet and supernatant were electrophoresed on 12% SDS-PAGE gels.
He et al. BMC Cell Biology 2009 10:81 doi:10.1186/1471-2121-10-81