G-actin in the presence of tau mutants at low speed co-sedimentation assay. Conditions were referred to Figure 2, G-actin (4 μg) was incubated with equal amount of tauRPD, tauMTBD, tau (panel a), and with tauΔPRD&MTBD (panel b) in binding buffer for 20 min in 37°C, followed by low-speed centrifugation. The pellet fraction (P) and supernatant fraction (S) were loaded in gels.
He et al. BMC Cell Biology 2009 10:81 doi:10.1186/1471-2121-10-81