High speed co-sedimentation assays of tauPRD and F-actin in the presence of NaCl. F-actin (at a constant amount) was incubated with different amounts of tauPRD (panel a) and tau (panel b) in binding buffer with 150 mM NaCl at 37°C for 40 min to mimic physiological ionic strength and then centrifuged (100,000 g, 4°C, 60 min). The pellet and supernatant were electrophoresed on 12% SDS-PAGE gels.
He et al. BMC Cell Biology 2009 10:81 doi:10.1186/1471-2121-10-81