Phosphorylation of CAP in SYF cells can be rescued upon expression of active forms of Src. Embryonic fibroblasts from the triple knock-out mouse for Src, Yes and Fyn (SYF cells) were transfected with CAP-EGFP together with various forms of Src kinase and stimulated with vanadate. No Tyr phosphorylation of CAP could be detected in cells expressing only CAP-EGFP or the inactive Src (Dead-Src). Phosphorylation of CAP was rescued upon coexpression of either c-Src or the constitutively active mutant Y527F-Src.
Fernow et al. BMC Cell Biology 2009 10:80 doi:10.1186/1471-2121-10-80