Figure 5.

Micronuclei generation after pRb acute loss in primary human fibroblasts. A) Bar graphs showing micronuclei percentages scored in RB transcriptionally silenced IMR90 cells (siRB) and in IMR90 cells with knock-down of both RB and AURORA-A or PLK1 compared with untransfected cells (untr.) or cells transfected with siRNA-luciferase (siLuc). B) Immunofluorescence microscopy analysis detecting the presence of centromeres (green spots) in IMR90 wild-type cells (b), in micronuclei generated following RB transcriptional silencing (c), and after knock-down of both RB and PLK1 (d) or after knock-down of both RB and AURORA-A (e). In (a) are shown lagging chromosomes (arrow) observed during metaphases in RB transcriptionally silenced cells. Nuclei were stained with DAPI (blue).

Amato et al. BMC Cell Biology 2009 10:79   doi:10.1186/1471-2121-10-79
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