Table 1

Receptor expression levels in HME cell lines

Clone

Order of gene transfer

Drug selection

EGFR (103/cell)

HER2 (103/cell)

HER3 (103/cell)


HER2-/3-

Parental

none

200a ± 8

30b

2c, d

HER2+/3-

HER2 into HER2-/3-

G418

151b ± 5

600a ± 19

2c, d

HER2-/3+

HER3 into HER2-/3-

Puromycin

200b ± 7

30b

28 ± 1c

HER2+/3+

HER3 into HER2+/3-

G418 and Puromycin

84b ± 7

625b ± 34

28 ± 3c


a An EGFR expression level of 200 K for the HER2-/3- cell line and a HER2 expression level of 600 K for HER2+/3- cell line were reported previously [42].

b These EGFR and HER2 expression levels were calculated by normalizing the receptor masses measured in ELISA experiments with the respective EGFR and HER2 expression levels shown in item (a) above.

c These HER3 expression levels were calculated by normalizing the receptor masses measured in ELISA experiments with the EGFR receptor expression level shown in item (a) above. Detected expression values were validated by re-computing them based on relative readings with respect to HER2 expression.

d This is also roughly the detection limit in our ELISA experiments.

Zhang et al. BMC Cell Biology 2009 10:78   doi:10.1186/1471-2121-10-78

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