Table 3

Mutants analyzed in this study

Mutant/WT

Sequence

Rescue


SBH1P TM

MLRVDPLVVLFLAVGFIFSVVALHVIS

Yes

SBH1P TM SP1

MLRVDSLVVLFLAVGFIFSVVALHVIS

Yes

SBH1P TM SP2

MLRVDPLVGLFLAVGFIFSVVALHVIS

Yes

SBH1P TM DP

MLRVDSLVGLFLAVGFIFSVVALHVIS

No

SBH1P TM mutant 1

MLRVDPLVVLFLAVGFTSSVVALHATS

Yes

SBH1P TM mutant 2

MLRVDPLVVLFLAVGFIFSVVSLHVIS

Yes

SBH1P TM mutant 3

MLRVDPLVVLLLAAGFILSVAASHVTS

Yes

SBH1P TM mutant 4

MLRVDPLVVLFPAVGFIFSVVALHVIS

Yes

SBH1P TM mutant 5

MLRVDPLVVLFLAVGSIFPVVALRVIS

No

SBH1P TM mutant 6

MLRVDPLVVLSLAVGFVFPVVALHVIS

No

SBH1P TM mutant 7

MLRVDPLVALFLAVGFNFSVVAPHVIS

No

SBH1P TM mutant 8

MLRVDPLVELFPAVGFISSVVALHVIS

No

SBH1P TM mutant 9

MLTVDPLVVLFLAVGFILSVVALHVIS

Yes

SBH1P TM mutant 10

MLRVDPLVGLLLAVGFILSVVALHVIS

Yes

SBH1P TM mutant 11

MLRVDPLVVLFLAVGFIPSVAALHVIS

Yes

SBH1P TM mutant 12

MLRVDPLAVSFLAAGFIPSVVALHVIS

Yes

SBH1P TM mutant 13

MLRVDPLAVLFPAVGFIYSVVALHVIS

Yes

SBH1P TM mutant 14

MLRVDPLVVLFLAVGFTFSVVALHVIS

Yes

SBH1P TM mutant 15

MLRVDPSVVLFLAVGLIFSVVALRVIS

Yes

SBH1P TM mutant 16

MPSVDPLVVLFPAVGFIFSVVALHVIS

Yes

SBH1P TM mutant 17

MLRVDPLVVLFLAVGFVFSVVALHVIS

Yes

SBH1P TM mutant 18

MLRVGPLVVLFLAVGFIFSVVALHVIS

Yes

SBH1P TM mutant 19

MLRVDPLVVLFLAVGSIFSVVALHVIS

Yes

SBH1P TM mutant 20

MLRVDPLVVLFLAVGFIFPVVALHVIS

Yes

SBH1P TM mutant 21

MLRVGPLVVLFLAVGLIFSVAALRVIS

No

SBH1P TM mutant 22

MPRVDPLVVLSLAVGFVFPVVALHVIS

No

SBH1P TM mutant 23

MLRADPLVALFLAVGSIFSVVALHVIS

No

SBH1P TM mutant 24

MPRVDPLVELFPAVGFISSVVALHVIS

No

SBH1P TM mutant 25

MPRVDPLVALFLAVGFNFSVVAPHVIS

No

SBH1P TM mutant 26

MLRVDPLVALFLAVGLTFSVVAYRYFL

No

SBH1P TM mutant 27

MLRVDPLAASFPAVGSYLPVAALHVIS

No

SBH1P TM mutant 28

MLRVDPLVASSLAVGLVFPVVALHVIS

No


Black color highlights the mutated amino acid

Zhao and J√§ntti BMC Cell Biology 2009 10:76   doi:10.1186/1471-2121-10-76

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