Table 2

Yeast expression vectors used in this study

Plasmid name

Insert

Marker

Source


pVT102U

-

URA3

[32]

YEpSBH1

SBH1(1-82)

URA3

This study

YEpSBH1-TM

SBH1(50-75)

URA3

This study

YEpBIO-(FL)SBH1

BIO-SBH1(1-82)

URA3

This study

YEpBIO-SBH1-TM

BIO-SBH1(50-75)

URA3

This study

YEpBIO-SBH1FL(DM)

BIO-SBH1(1-82, P54S V57G)

URA3

This study

YEpBIO-SBH1TM (DM)

BIO-SBH1(50-75, P54S V57G)

URA3

This study

YEpBIO-SBH1TM(SM1)

BIO-SBH1(50-75, P54S)

URA3

This study

YEpBIO-SBH1TM(SM2)

BIO-SBH1(50-75, V57G)

URA3

This study

YEpSBH1-FL(DM)L

BIO-SBH1(1-82, P54S V57G)

LEU2

This study

YEpSBH1-TM(DM)L

BIO-SBH1(50-75, P54S V57G)

LEU2

This study

YEpSBH1-TM(SM1)L

BIO-SBH1(50-75, P54S)

LEU2

This study

YEpSBH1-TM(SM2)L

BIO-SBH1(50-75, V57G)

LEU2

This study

p425ADH

-

LEU2

[35]

p426ADH

-

URA3

[35]

YEpBIO-SBH1TM-M1

BIO-SBH1(50-75, I65T F66S V73A I74T)

URA3

This study

YEpBIO-SBH1TM-M2

BIO-SBH1(50-75, A70S)

URA3

This study

YEpBIO-SBH1TM-M3

BIO-SBH1(50-75, F59L V62A F66L V69A L71S I74T)

URA3

This study

YEpBIO-SBH1TM-M4

BIO-SBH1(50-75, L60P)

URA3

This study

YEpBIO-SBH1TM-M5

BIO-SBH1(50-75, F64S S67P H72R)

URA3

This study

YEpBIO-SBH1TM-M6

BIO-SBH1(50-75, I65V S67P)

URA3

This study

YEpBIO-SBH1TM-M7

BIO-SBH1(50-75, V57A I65N L71P)

URA3

This study

YEpBIO-SBH1TM-M8

BIO-SBH1(50-75, V57E L60P F66S)

URA3

This study


All plasmids are 2 μ-type and contain the ADH1 promoter

Zhao and Jäntti BMC Cell Biology 2009 10:76   doi:10.1186/1471-2121-10-76

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