C-Tpr import into the nucleus is cytosol, temperature and energy dependent and is blocked by WGA. Digitonin-permeabilized NRK cells were incubated with 8 pmoles of Cy5-C-Tpr for 20 min at 30°C (panel A) or 4°C (panel B) in the presence of cytosol and an energy-regenerating system. Panel C: 8 μg WGA was added. Panel D: the ATP regenerating system was replaced by an ATP-depleting system, 0.8885 U hexokinase + glucose. Samples were visualized by confocal microscopy.
Ben-Efraim et al. BMC Cell Biology 2009 10:74 doi:10.1186/1471-2121-10-74