Matrix rigidity regulates cell surface area in primary oligodendrocytes. (A) Primary oligodendrocytes were cultured for 3-4 d on polyacrylamid gels of different rigidities and analyzed for the expansion of myelin membrane sheets by staining for MBP (green). Scale bar, 20 μm. (B) The relative sheet size of primary oligodendrocytes was quantified. Values represent the mean ± SEM (n > 60 cells, ***p < 0,001).
Kippert et al. BMC Cell Biology 2009 10:71 doi:10.1186/1471-2121-10-71