Figure 2.

Matrix rigidity regulates cell surface area and endocytosis in Oli-neu cells. (A) Quantification of the elastic shear moduli of polyacrylamid gels on glass coverslips with varying amounts of bisacrylamid. Values represent means ± SD (n > 30 measurements). (B) Oli-neu cells were cultured for 1 d on polyacrylamid gels of different rigidities (using bisacrylamid varying from 0,5% to 0,01%) and analyzed for surface area differences by staining with Alexa Flour 488 conjugated wheat germ agglutinin (green). Scale bar, 10 μm. (C) Quantification of the surface area of Oli-neu cells on polyacrylamid gels of different rigidities (mean ± SEM; n > 100 cells, ***p < 0,001). (D+E) Cells were cultured for 1 d on polyacrylamid gels of different rigidities by varying the amount of bisacrylamid. Changes in the amount of dextran uptake ((D) 15 min and (E) 30 min) were quantified. Values represent the mean ± SEM (n > 60 cells from three independent experiments, ***p < 0,001). (F) Quantification of cell surface area of Oli-neu cells cultured on polyacrylamid gels for 1 d and treated for 10 h with 10 μM Y27632 or C3 transferase (mean ± SEM; n > 100 cells, *p < 0,05, **p < 0,01, ***p < 0,001). (G) Quantitative analysis of surface area changes of Oli-neu cells cultured for 1 d on polyacrylamid gels and treated with 50 μM blebbistatin (blebb) for 10 h (mean ± SEM; n > 60 cells, ***p < 0,001). (H) Oli-neu cells were cultured on polyacrylamid gels of different rigidities, treated with RGD-peptide or inactive RAD-peptide and changes in relative surface area were quantified. Values represent the mean ± SEM (n > 80 cells, *p < 0,05, **p < 0,01, ***p < 0,001).

Kippert et al. BMC Cell Biology 2009 10:71   doi:10.1186/1471-2121-10-71
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