Email updates

Keep up to date with the latest news and content from BMC Cell Biology and BioMed Central.

Open Access Highly Accessed Research article

Actomyosin contractility controls cell surface area of oligodendrocytes

Angelika Kippert1, Dirk Fitzner12, Jonne Helenius3 and Mikael Simons12*

Author affiliations

1 Max-Planck-Institute for Experimental Medicine, Hermann-Rein-Str. 3, Göttingen, Germany

2 Department of Neurology, Robert-Koch-Str. 40, University of Göttingen, Göttingen, Germany

3 Deptartment of Cellular Machines, Biotechnology Center, University of Technology, Tatzberg 47-51, Dresden, Germany

For all author emails, please log on.

Citation and License

BMC Cell Biology 2009, 10:71  doi:10.1186/1471-2121-10-71

Published: 25 September 2009

Abstract

Background

To form myelin oligodendrocytes expand and wrap their plasma membrane multiple times around an axon. How is this expansion controlled?

Results

Here we show that cell surface area depends on actomyosin contractility and is regulated by physical properties of the supporting matrix. Moreover, we find that chondroitin sulfate proteoglycans (CSPG), molecules associated with non-permissive growth properties within the central nervous system (CNS), block cell surface spreading. Most importantly, the inhibitory effects of CSPG on plasma membrane extension were completely prevented by treatment with inhibitors of actomyosin contractility and by RNAi mediated knockdown of myosin II. In addition, we found that reductions of plasma membrane area were accompanied by changes in the rate of fluid-phase endocytosis.

Conclusion

In summary, our results establish a novel connection between endocytosis, cell surface extension and actomyosin contractility. These findings open up new possibilities of how to promote the morphological differentiation of oligodendrocytes in a non-permissive growth environment.

See related minireview by Bauer and ffrench-Constant: http://www.jbiol.com/content/8/8/78 webcite