Additional file 2.

Effect of incubation time on the readout. Three assay-positive constructs with various protein sizes (NANOG (305 aa), ARNT2 (717 aa) and CRIP1 (77 aa)) were selected from Additional File 4, then subjected to the assay with incubation times of 20, 30, and 40 h.

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Hoat et al. BMC Cell Biology 2009 10:69   doi:10.1186/1471-2121-10-69