Additional file 1.
Agarose gel electrophoresis of six ACT samples. (A) CDSs of six representative target genes with various ORF length were amplified by gene-specific forward and reverse primers and confirmed on 1.5% agarose gel (the first PCR). λ-HindIII and ϕX-HaeIII mix size marker was used. The expected bp sizes of 918 (NANOG), 2151 (ARNT2), 234 (CRIP1), 237 (NRGN), 417 (CDKN2B) and 1527 (FARSLA) were confirmed. (B) The first PCR products were directly applied to the second PCR to connect with the DNA fragments for CMV-TIP-1-TNNC2 and SV40 (ACT samples). The products were confirmed on 1.0% agarose gel (λ-StyI marker). The expected bp size (ORF + 2.1 kb) was confirmed for each ACT sample.
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Hoat et al. BMC Cell Biology 2009 10:69 doi:10.1186/1471-2121-10-69