Additional file 1.
RNAi of the newly identified cyclin and CRK homologues. The data shows the effect of RNAi on each individual novel cyclin and CRK. Procyclic form (29-13) cells were transfected with pZJM vectors carrying short (300-500 bp) fragments of the newly identified cyclin and CRK genes as indicated. Cell lines were selected using phleomycin and cloned. RNAi was induced by addition of tetracycline (10 μg/ml) and cell growth monitored daily using a hemocytometer. Insets show mRNA levels after RNAi for 2 days estimated by semi-quantitative RT-PCR. α-Tubulin was included as a loading control.
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Gourguechon and Wang BMC Cell Biology 2009 10:68 doi:10.1186/1471-2121-10-68