Analysis of basal body replication and segregation in CRK9 depleted cells. Cells induced for CRK9 RNAi for 5 days were fixed and stained with YL1/2 antibody to show the basal bodies. DAPI was used to stain the nuclei and kinetoplasts. Cells were classified as 1N1K1BB (N, nuclei, K, kinetoplast, BB, basal body), 1N1K2BB, 1N2K2BB or 2N2K2BB cells and tabulated from a population of ~200 cells in each sample (A). Phase and DAPI-stained images of three different types of cells, 1N1K1BB, 1N1K2BB and 1N2K2BB are shown in panel (B). The distances between the two basal bodies in ~200 1N1K2BB cells were measured using the ImageJ software and compared between the control and CRK9 depleted cells (C).
Gourguechon and Wang BMC Cell Biology 2009 10:68 doi:10.1186/1471-2121-10-68