Table 2

Ca2+ signaling parameters in ATP-stimulated COS-7 cells expressing either one of the mRFP-Tubby-domain, PLCδ1PH-mRFP and p130-PH-mRFP proteins

Construct

mRFP fluorescence

(AU)

T1/2 activation*

(sec)

N =


Tubby-domain

< 100

6.0 ± 0.3**

109

101-1000

6.0 ± 1.8

31

1001-5000

13.5 ± 3.5

40

5001-10000

12.0 ± 1.5

4


PLCδ1PH

< 100

3.5 ± 0.4

99

101-1000

6.5 ± 1.1

20

1001-5000

12.5 ± 3.9

34

5001-10000

18.0 ± 6.1

5


p130-PH

< 100

3.5 ± 0.4

91

101-1000

12.0 ± 4.9

16

1001-5000

19.5 ± 5.3

39

5001-10000

> 100

17


* The lag time of the Ca2+ response was defined as the time it takes for a cell to reach half-maximum of its Ca2+ peak after addition of 50 μM ATP.

**Values are calculated from cells expressing the mRFP-tagged proteins at the indicated intensities. Note the largest effects of the p130-PH-mRFP protein that does not bind to the membranes but binds InsP3 with comparable affinity to the PLCδ1PH-mRFP [27].

Szentpetery et al. BMC Cell Biology 2009 10:67   doi:10.1186/1471-2121-10-67

Open Data