Table 1

Localization responses of selected yeast PH domains and the human Tubby domain expressed in HEK293-AT1 or COS-7 cells.

PH domain

Subcellular

Localization

Response to

AngII stimulation

Response to

PtdIns(4,5)P2

elimination by

5-ptase

Presumptive inositide

dependence of

PM localization


PLCĪ“1-PH

PM

transient

translocation

complete

translocation

PtdIns(4,5)P2

Cla4p-PH

PM

no change

no change

inositide independent

Num1p-PH

PM

transient

translocation

complete

translocation

PtdIns(4,5)P2

Skm1p-PH

PM

no change

no change

inositide independent

Opy1p-PH

PM

transient

translocation

complete

translocation

PtdIns(4,5)P2

Yil105c/

Slm1p-PH

PM

transient

translocation

partial

translocation

PtdIns(4,5)P2

PtdIns4P

Ynl047/

Slm2p-PH

PM

no change

no change

inositide independent

Tubby domain

PM

transient

translocation

complete

translocation

PtdIns(4,5)P2


Szentpetery et al. BMC Cell Biology 2009 10:67   doi:10.1186/1471-2121-10-67

Open Data