Comparison of gene expression in Perk deficient islets and 832/13 transformed beta cells. The expression of mRNAs in Perk-/- islets and AdDNPerk 832/13 beta cells were normalized to Perk+/+ islets and AdLacZ 832/13 beta cells, respectively. For the 832/13 cells a low dose of adenovirus (MOI = 5) was used, and the data from 2 to 5 independent experiments were analyzed. For islets, mRNA expression was also quantified from 5-9 WT and 4-9 Perk KO mice. For Xbp-1 the spliced Xpb-1 mRNA form was normalized to total Xbp-1 mRNA. Not assayed for specific mRNAs denoted as n.a.
Feng et al. BMC Cell Biology 2009 10:61 doi:10.1186/1471-2121-10-61