Additional file 1.
Full-length zyxin and deletion mutant zyxin (hZyx 1–378 aa (Δ1)). The data show nonspecific binding of full-length zyxin to GST and/or glutathione-sepharose beads and no signals for the product of hZyx 1–378 aa (Δ1). HEK293T cells expressing the full-length or indicated deletion mutant of zyxin were lysed and the resulting lysates were incubated with GST-SIRT1 or control GST proteins immobilized on glutathione sepharose beads. Bound proteins were probed with anti-HA antibody (upper left panel). HA-zyxin or its mutants in lysates were revealed by immunoblotting with anti-HA antibody (upper right panel). The amounts of GST-SIRT1 and control GST proteins were revealed by amido black staining (lower panel).
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Fujita et al. BMC Cell Biology 2009 10:6 doi:10.1186/1471-2121-10-6