Additional file 5.
Effect of co-overexpression of Arf1T31N and Arf6T27N on the appearance of Pex14p-immunoreactive particles in Ptk2 cells. Ptk2 cells were transiently transfected with a plasmid coding for Arf1T31N-HA and a bicistronic plasmid encoding EGFP-PTS1 together with Arf6T27N. After 36 hours, the cells were fixed and processed for fluorescence analysis. The top row shows three images of the signals observed for endogenous Pex14p (see Additional file 4, lower panels). The insets show an enlargement of the outlined regions. +, cell co-overexpressing Arf1T31N and Arf6T27N; -, cell overexpressing only Arf6T27N; 0, non-transfected cell (for overview images, see Additional file 4). Scale bar: 20 μm.
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Anthonio et al. BMC Cell Biology 2009 10:58 doi:10.1186/1471-2121-10-58