Open Access Research article

Evaluation of expression and function of the H+/myo-inositol transporter HMIT

Elena Di Daniel1*, Man HS Mok1, Emma Mead1, Chiara Mutinelli2, Erika Zambello2, Laura L Caberlotto2, Theresa J Pell1, Christopher J Langmead1, Ajit J Shah4, Graham Duddy3, James NC Kew1 and Peter R Maycox1

Author Affiliations

1 Psychiatry Discovery Technology Group, GlaxoSmithKline, New Frontiers Science Park, Harlow, UK

2 Psychiatry Discovery Technology Group, GlaxoSmithKline, New Frontiers Science Park, Verona, Italy

3 Core Discovery Technology Group, GlaxoSmithKline, New Frontiers Science Park, Harlow, UK

4 Psychiatry Centre of Excellence for Drug Discovery, GlaxoSmithKline, New Frontiers Science Park, Harlow, UK

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BMC Cell Biology 2009, 10:54  doi:10.1186/1471-2121-10-54

Published: 16 July 2009

Additional files

Additional file 1:

Additional methods and additional figure legends. Details of additional methods and figure legends provided.

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Additional file 2:

Anti-HMIT antibody validation. Western blotting of HEK293 cell lysates following immunoprecipitation; HMIT immunocytochemistry in transfected HEK293 cells, HMIT immunoprecipitation and Western blotting in rat and mouse brain tissue.

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Additional file 3:

Analysis of rat saggital slices stained with anti-HMIT:21 antibody. Results of infra-red analysis of HMIT localisation in the rat brain.

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Additional file 4:

Generation of HMIT null-mutant mice. Description of strategy used to generate HMIT null-mutant mice and mRNA analysis in neurons.

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