Additional file 1.
Inhibitory effects of BQ123 and U0126 on pERK1/2 activity in HASMCs. The data provided represent the Western Blot analysis of inhibitory effects of ETA receptor inhibitor BQ123 and MEK inhibitor U0126 on ET-1-induced phosphorylation of ERK1/2. 24 h Serum-starved cells were stimulated with 10 nM of ET-1 for 10 min after cells were treated with inhibitors for 30 min. Phosphorylated ERK1/2 activity was determined by western blot with an anti-phospho-ERK1/2 antibody, and presented as a relative extent to the level of β-actin. A, bar graph shows inhibitory effects of 5 μM of ETA/ETB receptor inhibitors on phosphorylated ERK1/2 activity induced by ET-1. B, bar graph shows inhibitory effect of 1 μM of U0126 on phosphorylated ERK1/2 activity induced by ET-1. The upper panels of A and B indicate representative autoradiographs of western blot showing phosphorylated ERK1/2 and β-actin. Data represent mean ± S.E.M. * p < 0.05, ** p < 0.01 compared with the ET-1-stimulated states after DMSO treatment. p = phosphorylation, ns = non-significant.
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Chen et al. BMC Cell Biology 2009 10:52 doi:10.1186/1471-2121-10-52