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Evidence for a mitochondrial localization of the retinoblastoma protein

Ioana Ferecatu12, Nathalie Le Floch12, Marie Bergeaud1, Aida Rodríguez-Enfedaque12, Vincent Rincheval1, Lisa Oliver3, François M Vallette3, Bernard Mignotte12 and Jean-Luc Vayssière12*

Author Affiliations

1 Laboratoire de génétique et biologie cellulaire – CNRS UMR 8159, Université de Versailles Saint-Quentin-en-Yvelines, Versailles, France

2 Laboratoire de génétique moléculaire et physiologique, Ecole Pratique des Hautes Etudes, Versailles, France

3 INSERM U601, Faculté de Médecine – Université de Nantes, Nantes, France

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BMC Cell Biology 2009, 10:50  doi:10.1186/1471-2121-10-50

Published: 25 June 2009

Additional files

Additional file 1:

Nuclear pattern of Rb in the immunofluorescence study. The image provided represents the absence of the mitochondrial pattern of Rb protein in the immunofluorescence studies when using the G3-245 antibody. A. Untreated human fibrosarcoma HT1080 cells were stained with anti-RbIF8 antibodies (in red) and the nuclei were labeled with Hoechst (in blue). The superimposition of Rb with nuclei is detected in pink in the overlay image. B. The same cells were stained with anti-RbG3-245 antibodies (in red); co-stained with mitochondrial marker anti-ANT (in green) and the nuclei were labeled with Hoechst (in blue). No superimposition is detected in the overlay image (no yellow color is visualized).

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