mRNA expression levels of endocrine and exocrine markers. Insulin, chromogranin, SUR1, PCSK1, PCSK2, glucagon, and amylase were measured by Real-time RT-PCR reaction using SYBR® Green Dye reagent (Applied Biosystems, Foster City, CA, USA). All reactions were performed in triplicates (n = 4 independent experiments). The relative expression of a target gene was determined in comparison to a reference gene (HPRT). In addition H-MBS mRNA-expression level was used as a positive control for similar expression levels between cell lines and human islets. Results are presented as relative increase where the value obtained from primary cultures of human islets was set to 100. a p < 0.001. APM, VGA or CPR cells vs. human islets. For SUR1 and PCSK1mRNA levels: a p < 0.001. APM, VGA or CPR cells vs. human islets, and also CPR vs. APM or VGA cells. ANOVA/Bonferroni's tests.
Labriola et al. BMC Cell Biology 2009 10:49 doi:10.1186/1471-2121-10-49