Validation of the interaction of TWIST with TCF-4 using immunoprecipitation assays. (A) Expression of wild type TWIST was shown by Western blotting using the anti-V5 antibody, (B) HEK293 cells were co-transfected with plasmids expressing V5-HIS-TWIST and CBP-TCF-4. Two different CBP antibodies were used (clone ID; K-24 and W-15). W-15 was used for the immunoblot. IgG beads with protein and antibody, and beads without antibody were used as positive and negative controls, respectively. Nuclear extracts were immunoprecipitated with anti-CBP tag antibodies (K-24 and W-15) bound resin, and the precipitated proteins were detected using anti-V5 antibody.
Singh and Gramolini BMC Cell Biology 2009 10:47 doi:10.1186/1471-2121-10-47