Additional file 2.
The 53 kDa-form is the major form of p53 in mouse ES cells. (A) R1, D3 and 3T3 cells, for control, were lysed and 50 μg of the proteins were separated by a 10% SDS-PAGE gel. The proteins were blotted onto an Immobilone P membrane and hybridised with the polyclonal anti-p53 antibody CM5. After washing and hybridisation with an anti-rabbit antibody coupled to HRP, the blot was developed by ECL. (B) Total RNA was prepared from D3, R1 and 3T3 cells, for control, and transcribed into cDNA. PCR was performed using gene specific primers for murine p53 and analysed by agarose gel electrophoresis.
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Solozobova et al. BMC Cell Biology 2009 10:46 doi:10.1186/1471-2121-10-46