Additional file 1.
Cytoplasmic localization of p53 in ES cells. R1, D3 and CGR8 mouse embryonal stem cells were plated on cover slips (R1 and D3 in the presence of feeders, CGR8 in the absence of feeders). Two days after plating, cells were fixed in acetone/methanol, permeabilized with Triton-X-100 and incubated with the anti-p53 antibody Pab 421 or only with blocking buffer, for control. After incubation, cover slips were washed and incubated with an antibody directed against mouse IgG, coupled to Alexa-Fluor-488 (green). To visualize the nuclei, cover slips were incubated with Draq5 (blue).
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Solozobova et al. BMC Cell Biology 2009 10:46 doi:10.1186/1471-2121-10-46