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High-content live cell imaging with RNA probes: advancements in high-throughput antimalarial drug discovery

Serena Cervantes1, Jacques Prudhomme2, David Carter3, Krishna G Gopi4, Qian Li6, Young-Tae Chang45 and Karine G Le Roch2*

Author Affiliations

1 Cell, Molecular, and Developmental Biology Graduate Program, University of California, Riverside, CA, 92521, USA

2 Department of Cell Biology and Neurosciences, University of California, Riverside, CA, 92521, USA

3 Institute for Integrative Genome Biology, University of California, Riverside, CA, 92521, USA

4 Department of Chemistry, & NUS MedChem Program of the Office of Life Sciences, National University of Singapore, 11754, Singapore

5 Laboratory of Bioimaging Probe Development, Singapore Bioimaging Consortium, Agency for Science, Technology and Research (A*STAR), Biopolis, 138667, Singapore

6 Department of Chemistry, New York University, New York, NY 10003, USA

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BMC Cell Biology 2009, 10:45  doi:10.1186/1471-2121-10-45

Published: 10 June 2009

Additional files

Additional file 1:

DNA: RNA binding assay. Graph represents 132A staining of RNA and DNA in a mixed population, with one nucleic acid kept at a steady state and the other serially diluted, where the maximum fluorescence intensity was set to one. Steady state RNA was observed to have a higher fluorescence intensity than serially diluted RNA, indicating 132A has a higher fluorescent signal when bound to RNA.

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Additional file 2:

Merged montage images. A tiling of two by two images of transmitted light and fluorescent parasites, were taken with the Pathway HT. Decreased parasitemia with increasing concentration of chloroquine was observed with the 3D7 strain.

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