Comparison of SUV420H2 and H2AFY dynamics by FRAP. (A) Cellular localization of GFP-H2AFY fusion proteins stably expressed in L929 cells. Cells were fixed with paraformaldehyde and localization of fusion proteins was visualized with fluorescence microscopy. GFP-H2AFY fusion proteins colocalize with dense foci identified by Hoechst staining as pericentric heterochromatin regions. (B) Time-lapse series of confocal images of living cells expressing GFP-SUV420H2 and GFP-H2AFY as indicated, during FRAP experiments. Images were recorded before (Prebleach) and at different time intervals after the bleach. Arrows indicate the photobleached areas. (C) Protein dynamics derived from FRAP curves. The percentage of fluorescence recovery after 20 min, and the number of FRAP curves used are indicated.
Souza et al. BMC Cell Biology 2009 10:41 doi:10.1186/1471-2121-10-41