Effect of PD98059 (PD), U0126, U0124 and LY294002 (LY) on GM-CSF upregulated TLR3 (A) and TLR7 (B) expression in P815 cells. Cells were preincubated with PD (50 μM), U0126 (5 μM), U0124 (5 μM) or LY (20 μM), respectively for 30 min before GM-CSF (10 and 100 ng/ml) being added for 6 h at 37°C. Values shown are Mean ± SE for four separate experiments performed in duplicate. * P < 0.05 compared with the response to corresponding uninhibited control.
Yang et al. BMC Cell Biology 2009 10:37 doi:10.1186/1471-2121-10-37