Figure 5.

Effect of PD98059 (PD), U0126, U0124 and LY294002 (LY) on GM-CSF induced IL-13 (A) and IL-6 (B) secretion from P815 cells. Cells were preincubated with PD (50 μM), U0126 (5 μM), U0124 (5 μM) or LY (20 μM), respectively for 30 min before GM-CSF (10 and 100 ng/ml) being added for 6 h at 37°C. Values shown are Mean ± SE for four separate experiments performed in duplicate. * P < 0.05 compared with the response to corresponding uninhibited control.