Figure 5.

Immunocytochemistry of tRPE cells expressing cytoskeletal proteins. A) Vimentin immunocytochemistry of tRPE cells grown on BCE-ECM using conditioned media. The red photograph was taken through the TRITC filter cube showing the finger-like projections of vimentin, which was overlayed with a blue photograph taken through the DAPI filter cube showing the nuclear counterstain Hoechst 33258. B) Pan-cytokeratin immunocytochemistry of tRPE cells grown on BCE-ECM using conditioned media. The green photograph was taken through the FITC filter cube showing the localization of the cytokeratin, and overlayed with a blue photograph taken through the DAPI filter cube showing the nuclear counterstain Hoechst 33258.

Liggett et al. BMC Cell Biology 2009 10:33   doi:10.1186/1471-2121-10-33
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