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## Figure 3.
Both CSN2 and CSN5 knockdowns result in reduced cell growth. K562 cells were transiently co-transfected with HKK plasmid together with either
shVC, shCSN2 or shCSN5 plasmid. HKK positive cells were sorted 24 hours post-transfection
and cells re-cultured. (A) Cell counts were taken daily and the cumulative growth
calculated. The cumulative growth of shCSN2 and shCSN5 cells is shown relative to
shVC. The insert graph has a different Y scale to highlight the differences between
shCSN2 and shCSN5 cumulative growth profiles. Data shown are the mean ± s.e.m. of
n = 3. * indicates a significant difference to shVC cell growth with a p value of
less than 0.05. (B) Thymidine incorporation in shCSN2 cells relative to shVC cells
was measured day 3, 5 and 7 post transfection. Data shown are the mean ± s.e.m. of
n = 3. (C) Thymidine incorporation in shCSN5 cells relative to shVC cells was measured
day 3 and 4 post transfection. Data shown are the mean ± s.e.m. of n = 3. * indicates
significance with a p value of less than 0.05.
Pearce |