The effect of compounds 1 (AI) and 2 (AII) on BAEC invasion into a Matrigel layer was studied using the chemoinvasion assay described below. Cells (1.7 × 104) were added to the Matrigel coated upper Boyden chamber and the compounds with or without FGF-2 added to the lower chamber. Cell invasion was measured after 24 h and assessed as described below. Values significantly different (p < 0.05) from FGF-2 alone are shown by *. Results are the mean of three experiments. (B) Representative photomicrographs are shown for the effect of compound 1 on BAEC (original magnification × 20). Increased migration was seen with FGF-2 (25 ng/ml; panel II) and this was reduced in the presence of compound 1 (panel IV).
Hussain et al. BMC Cell Biology 2009 10:30 doi:10.1186/1471-2121-10-30