Effect of stilbene glycosides on FGF-2 induced BAEC migration. (A) A cell monolayer was wounded as described below and recovery was assessed in the presence of increasing concentration of the test compounds. Results are shown for compound 1. Values significantly different from FGF-2 alone (p < 0.05) are shown by *. (B) Representative photomicrographs (original magnification × 20) show the effect of compound 1 on BAEC migration. DMSO was used as the control. (C) The effect compound 1 on FGF-2-induced cell migration in a three dimensional Boyden chamber assay. Cells were added to the upper chamber and the compound at a range of concentrations, with and without FGF-2 (25 ng/ml) added to the lower chamber (see below for method). The total number of migrated cells to the lower chamber was counted as described below. Column 1, control. The results are the mean of three experiments and values significantly different from FGF-2 alone (p < 0.05) are shown by *.
Hussain et al. BMC Cell Biology 2009 10:30 doi:10.1186/1471-2121-10-30