The centrosome protein PCM-1 is part of a detergent-resistant perinuclear matrix in differentiated muscle cells. (A) Purified nuclei from differentiated C2C12 cells were incubated for 10 minutes in buffer, containing as indicated, 0.1% Triton X-100, or 1% Triton X-100, or 1.5 M NaCl, or 6 M urea, or 8 M urea, or 2% sodium dodecyl sulfate. For details, see 'Methods'. Nuclei were spun onto coverslips and processed for immunofluorescence of PCM-1. DNA was stained with 4',6-diamidino-2-phenylindole. Bar, 10 μm. (B) Percentage of nuclei that stained positively for perinuclear PCM-1, following treatment as specified in (A). A minimum of three experiments were performed, counting more than 100 nuclei per experiment.
Srsen et al. BMC Cell Biology 2009 10:28 doi:10.1186/1471-2121-10-28