AhR agonist TCDD and AhR antagonist RSV regulated MMP-9 expression and activity in AGS cells. After different concentrations (as shown above) of TCDD treatment for 24 hours, (A) RT-PCR analysis of MMP-9 mRNA expression in a concentration-response. (B) Gelatin zymography analysis of MMP-9 protein activity in a concentration-response. After co-treatment with TCDD (1 nM) and RSV (at different concentrations as shown above) for 24 hours, (C) mRNA expression of MMP-9 was detected by RT-PCR. (D) Protein activity of MMP-9 was detected by gelatin zymography.
Peng et al. BMC Cell Biology 2009 10:27 doi:10.1186/1471-2121-10-27