Additional file 2.

In response to a Ca2+ stimulus, EGFP-NEDD4L-C2(+) does not target the endoplasmic reticulum, lysosome, Golgi complex, mitochondria, early endosome or autophagosome. Confocal images of live A6 cells transiently transfected with EGFP-NEDD4L-C2(+), treated with 10 uM ionomycin and 1.97 mM Ca2+, and incubated in the presence of ER-Tracker™ Red (A), Lysotracker® Red (B), BODIPY® TR C5-ceramide complexed to BSA (Golgi marker) (C), MitoTracker® Red (D), or Transferrin-Texas Red® (E). A confocal image of an A6 cell that was transiently cotransfected with EGFP-NEDD4L-C2(+) and mCherry-LC3 (autophagosome marker) after a Ca2+ stimulus (F). Blue, green and red channel overlay (A-F). Scale bars are equivalent to 10 μm.

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Garrone et al. BMC Cell Biology 2009 10:26   doi:10.1186/1471-2121-10-26