Distinct subcellular localization behaviors of EGFP tagged α- and β-ENaC and mCherry tagged NEDD4L-C2(+) or NEDD4L-C2(+)-C943A in response to an ionomycin treatment in the presence of Ca2+. Confocal images of live A6 cells stably expressing α-ENaC-EGFP (A-E) or EGFP-β-ENaC (F-J, K-O) transiently transfected with mCherry-NEDD4L-C2(+) (A-E, F-J) or mCherry-NEDD4L-C2(+)-C943A (K-O) and incubated in 1.97 mM Ca2+. The images were acquired at 0 (A), 30 (B), 210 (C), 420 (D, E), 0 (F), 41 (G), 66 (H), 236 (I, J), 0 (K), 80 (L), 120 (M), and 240 (N, O)seconds after a 10 μM ionomycin addition. All images are overlays of the blue, green and red channels except for images E, J, and Owhich show only the blue and green channels.
Garrone et al. BMC Cell Biology 2009 10:26 doi:10.1186/1471-2121-10-26