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GC-1 mRHBDD1 knockdown spermatogonia cells lose their spermatogenic capacity in mouse seminiferous tubules

Yong Wang1, Wei Song1, Shuchun Li1, Xin Guan1, Shiying Miao1, Shudong Zong2, SS Koide3 and Linfang Wang1*

Author affiliations

1 State Key Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, Peking Union Medical College, Tsinghua University 5 Dong Dan San Tiao, Beijing 100005, PR China

2 National Research Institute for Family Planning Beijing, WHO Collaboration Center of Human Reproduction 12 Da Hui Si, Hai Dian, Beijing 100081, PR China

3 Center for Biomedical Research, the Population Council, 1230 York Avenue, New York, NY 10021, USA

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Citation and License

BMC Cell Biology 2009, 10:25  doi:10.1186/1471-2121-10-25

Published: 10 April 2009



Apoptosis is important for regulating spermatogenesis. The protein mRHBDD1 (mouse homolog of human RHBDD1)/rRHBDD1 (rat homolog of human RHBDD1) is highly expressed in the testis and is involved in apoptosis of spermatogonia. GC-1, a spermatogonia cell line, has the capacity to differentiate into spermatids within the seminiferous tubules. We constructed mRHBDD1 knockdown GC-1 cells and evaluated their capacity to differentiate into spermatids in mouse seminiferous tubules.


Stable mRHBDD1 knockdown GC-1 cells were sensitive to apoptotic stimuli, PS341 and UV irradiation. In vitro, they survived and proliferated normally. However, they lost the ability to survive and differentiate in mouse seminiferous tubules.


Our findings suggest that mRHBDD1 may be associated with mammalian spermatogenesis.